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Chronic EtOH induces gut permeability, hepatic injury & inflammation. (A, B) Indirect makers of intestinal barrier dysfunction: Serum LPS and zonulin. (C, D) Serum markers of hepatic injury: ALT and AST. (E–I) Liver pro‐inflammatory markers: TNFα and IL‐6 protein expression, and the acute‐phase protein serum amyloid A quantified using a <t>colorimetric</t> ELISA (Panel H; gray outlines indicate standards; blue, control; orange, EtOH). Statistical analyses included unpaired t ‐tests or Welch's t ‐tests. *Significantly different from control group ( p < 0.05). Data are presented as mean ± SEM. Samples sizes were 6–7 for controls and 8–9 for EtOH.
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Chronic EtOH induces gut permeability, hepatic injury & inflammation. (A, B) Indirect makers of intestinal barrier dysfunction: Serum LPS and zonulin. (C, D) Serum markers of hepatic injury: ALT and AST. (E–I) Liver pro‐inflammatory markers: TNFα and IL‐6 protein expression, and the acute‐phase protein serum amyloid A quantified using a <t>colorimetric</t> ELISA (Panel H; gray outlines indicate standards; blue, control; orange, EtOH). Statistical analyses included unpaired t ‐tests or Welch's t ‐tests. *Significantly different from control group ( p < 0.05). Data are presented as mean ± SEM. Samples sizes were 6–7 for controls and 8–9 for EtOH.
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Chronic EtOH induces gut permeability, hepatic injury & inflammation. (A, B) Indirect makers of intestinal barrier dysfunction: Serum LPS and zonulin. (C, D) Serum markers of hepatic injury: ALT and AST. (E–I) Liver pro‐inflammatory markers: TNFα and IL‐6 protein expression, and the acute‐phase protein serum amyloid A quantified using a colorimetric ELISA (Panel H; gray outlines indicate standards; blue, control; orange, EtOH). Statistical analyses included unpaired t ‐tests or Welch's t ‐tests. *Significantly different from control group ( p < 0.05). Data are presented as mean ± SEM. Samples sizes were 6–7 for controls and 8–9 for EtOH.

Journal: Comprehensive Physiology

Article Title: Systemic Consequences of Chronic Ethanol Intake: From Microbiome Shifts to Metabolic Impairment

doi: 10.1002/cph4.70132

Figure Lengend Snippet: Chronic EtOH induces gut permeability, hepatic injury & inflammation. (A, B) Indirect makers of intestinal barrier dysfunction: Serum LPS and zonulin. (C, D) Serum markers of hepatic injury: ALT and AST. (E–I) Liver pro‐inflammatory markers: TNFα and IL‐6 protein expression, and the acute‐phase protein serum amyloid A quantified using a colorimetric ELISA (Panel H; gray outlines indicate standards; blue, control; orange, EtOH). Statistical analyses included unpaired t ‐tests or Welch's t ‐tests. *Significantly different from control group ( p < 0.05). Data are presented as mean ± SEM. Samples sizes were 6–7 for controls and 8–9 for EtOH.

Article Snippet: Serum lipid profiles including free fatty acids (FFAs), triglycerides, and total cholesterol were measured using enzymatic colorimetric assay kits (FFA: Cat # 102965‐238, Cell Biolabs; triglycerides: Cat # MA‐TG, Ray Bio Laboratories; and total cholesterol: Cat # EHDL004, BioAssay Systems) per the manufacturers' protocols.

Techniques: Permeability, Expressing, Enzyme-linked Immunosorbent Assay, Control